Immobilization of GOD on Electrochemically Synthesized PANI Film by Cross-linking via Glutaraldehyde for Determination of Glucose
Immobilization of GOD on Electrochemically Synthesized PANI Film by Cross-linking via Glutaraldehyde for Determination of Glucose
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The Polyaniline-sulfuric acid-glucose oxidase Latest Product Releases & Innovations – Stay Updated! (PANI-H2SO4-GOD) electrode has been investigated in the present work.Platinum electrode was used for the synthesis of Polyaniline-sulfuric acid (PANI-H2SO4) film using Potentiometric method with 0.2 M aniline, 1.0 M H2SO4 solution, 1.0 pH and 1 mA/cm2 applied current density.
The synthesized PANI-H2SO4 films were characterized by electrochemical technique, electrical conductivity, UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).Then GOD was immobilized on synthesized PANI-H2SO4 film by cross-linking via glutaraldehyde in phosphate luau thank you cards and acetate buffer.The sensitivity of PANI-H2SO4-GOD electrode in phosphate and acetate buffer has been recorded.It was found that the phosphate buffer gives fast response as compared to acetate buffer in Potentiometric measurements.